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Chemical Indicators: The Complete List

In teaching labs, quality control, or DIY water testing, choosing and understanding indicators makes routine checks far more reliable. A quick, organized list helps you compare which dyes respond at the pH or ion range you care about and where they’re commonly used.

There are 41 Chemical Indicators, ranging from Alizarin S to Xylenol orange. For each entry you’ll find below the data organized in the columns Type, Trigger / range, Typical uses so you can scan reactivity, operative pH/ion windows, and common applications at a glance — you’ll find below.

How do I pick the right indicator for my experiment?

Choose an indicator whose trigger/range overlaps the pH or concentration change you expect; check the Type to see if it’s acid–base, redox, or complexometric, and prefer indicators with clear color shifts and documented Typical uses similar to your setup to avoid ambiguous endpoints.

Are there common handling or storage tips for these indicators?

Store dyes in cool, dark conditions in tightly sealed containers to slow degradation; follow safety data sheets for solvent compatibility and disposal, and prepare fresh working solutions when color response appears weak or inconsistent.

Chemical Indicators

Name Type Trigger / range Typical uses
Phenolphthalein acid–base pH 8.2–10.0 Classic titrations (strong acid vs weak base), lab endpoint detection
Methyl orange acid–base pH 3.1–4.4 Titrations involving strong acid vs strong base or strong acid vs weak base
Bromothymol blue acid–base pH 6.0–7.6 pH indicator in biology, seawater testing, classroom demonstrations
Litmus acid–base,natural pH ~4.5–8.3 (acid/alkaline switch) General pH paper, quick school tests, field strips
Universal indicator acid–base pH 1.0–14.0 (mixture shows multi-color scale) pH paper, education, broad-range testing
Methyl red acid–base pH 4.4–6.2 Titrations (weak base vs strong acid), microbiology (MR test)
Thymol blue acid–base pH 1.2–2.8 and 8.0–9.6 (two transitions) Dual-range titrations, lab demonstrations
Bromocresol green acid–base pH 3.8–5.4 Protein assays, pH paper, titrations near pH 4.5
Phenol red acid–base pH 6.8–8.4 Cell culture media pH indicator, aquaria, titrations
Congo red acid–base pH ~3.0–5.0 Histology, amyloid staining, low-range pH tests
Nitrazine yellow acid–base pH 5.0–8.0 Medical pH tests (amniotic fluid), acid–base monitoring
Alizarin yellow R acid–base,complexometric pH 10.1–12.0 (pH indicator) / forms complexes with Al/Ca Analytical pH checks, metal detection
Thymolphthalein acid–base pH 9.3–10.5 Alkaline titrations, laboratory endpoint indicator
Eriochrome Black T complexometric Changes color when bound to Ca/Mg ions; free form wine-red, complexed blue EDTA titrations for water hardness (Ca/Mg), teaching labs
Calmagite complexometric Color change on binding Ca/Mg (typically red→blue) EDTA titrations for water hardness, metal assays
Murexide complexometric Forms violet complexes with calcium; color shifts with pH Calcium titrations, pharmaceutical analysis
Alizarin complexone complexometric Forms colored complexes with Ca/Al; indicator in EDTA titrations Complexometric titrations for divalent/trivalent metals, soil/plant testing
PAR (4-(2-pyridylazo)resorcinol) complexometric Forms colored complexes with many metal ions (Fe, Cu, Zn) Colorimetric metal assays, spectrophotometric detection, spot tests
Xylenol orange complexometric Indicator for metal-EDTA titrations; color changes with complex formation Trace metal determination, EDTA titrations
Ferroin redox Redox transition observable with Fe2+/Fe3+; color changes from red (reduced) to pale blue (oxidized) Redox titrations (e.g., ceric sulfate), Fe2+/Fe3+ monitoring
Dichlorophenolindophenol (DCPIP) redox Blue (oxidized) → colorless (reduced) Vitamin C (ascorbic acid) titration, photosynthesis assays
Starch (iodine-starch complex) redox Forms deep blue/black complex with iodine (triiodide), sensitive endpoint Endpoint indicator in iodine titrations, redox assays
Methylene blue redox Blue (oxidized) ↔ colorless (reduced) Redox titrations, biological redox assays, oxygen indicator
Diphenylamine redox Turns blue with strong oxidizers (e.g., nitrates under acid) Nitrate detection, oxidizer spot tests
Resazurin redox Blue (oxidized) → pink → colorless (reduced) Microbiology viability assays, redox-state monitoring in food and water
Fluorescein (Fajans adsorption) precipitation/adsorption Adsorbs on precipitate surface producing a fluorescent/visible color shift Fajans titration endpoints (chloride by silver), adsorption indicators
Potassium chromate (Mohr method) precipitation Chromate forms red-brown Ag2CrO4 precipitate at chloride endpoint Chloride titrations by silver nitrate (Mohr titration)
Phenolphthalein-methyl orange mixed strips acid–base Broad pH band coverage (strip shows multiple colors) pH test strips for field or classroom use
Alizarin S complexometric Forms colored complexes with aluminum and other metals; pH-dependent color shifts Aluminum detection, complexometric assays
PAN (1-(2-pyridylazo)-2-naphthol) metal ion Forms colored complexes with metals (Cu, Fe, Zn) visible to eye Spot tests, field metal detection, colorimetric assays
Curcumin (turmeric) natural,acid–base Yellow → red-brown in alkaline conditions (pH ~8–10) Home pH tests, natural indicator demonstrations
Red cabbage extract (anthocyanins) natural,acid–base pH 2–14 produces colors purple→red→green→yellow DIY pH indicator strips, classroom demonstrations
Beetroot (betalains) natural,redox/acid–base Color shifts with pH and oxidative conditions; red pigments bleach on oxidation Food testing, educational demonstrations
Ferricyanide/ferrocyanide redox indicators redox Color change when oxidized/reduced (complex dependent) Redox sensing, electrochemistry demonstrations
Tollen’s reagent (silver mirror) precipitation/redox Reduction of Ag+ to metallic silver produces mirror/film Aldehyde tests (qualitative), school demonstrations
Stannous chloride/iodine test redox/precipitation Iodine liberated or reduced produces visible color/precipitate Iodometric assays, halide detection, redox spot tests
Fluorescent pH indicators (e.g., SNARF dyes) acid–base pH-dependent emission wavelength/intensity changes Microscopy pH imaging, cell biology assays
Lead(II) chromate (spot tests) precipitation/metal ion Forms colored precipitate with certain anions/ions Qualitative spot tests for sulfates/chlorides in non-quantitative assays
Oxazine dyes (redox) redox Color changes on reduction/oxidation visible by eye Redox indicators in biochemical assays and titrations
Xylenol blue (sulfonephthalein type) acid–base pH 6.6–8.0 (example range varies by dye) pH indicator in buffers, clinical tests, titrations
Coomassie Brilliant Blue (protein dye with pH sensitivity) acid–base/protein binding Color shifts when binding proteins (blue intensity change) Protein assays (Bradford) where binding produces color change

Images and Descriptions

Phenolphthalein

Phenolphthalein

Phenolphthalein is colorless in acid and turns pink–fuchsia above pH 8.2. Favored for clear endpoints in many titrations, inexpensive and reliable; not suitable near neutral or physiological pH. Handle as a mild irritant; soluble in alcohol.

Methyl orange

Methyl orange

Methyl orange shifts from red in acid to yellow in base around pH 3.1–4.4. It gives sharp endpoints for titrations where phenolphthalein is unsuitable. Use cautiously—acidic solutions give the clearest color change.

Bromothymol blue

Bromothymol blue

Bromothymol blue changes from yellow in acid to blue in base with green near neutral (pH 6.0–7.6). Common for biological pH monitoring and demonstrations; easy-to-see midrange transition. Store protected from strong light.

Litmus

Litmus

Litmus is a traditional lichen-derived indicator: red in acid, blue in base. Used in paper strips and quick checks. Robust and easy to use, but gives only broad acidic/alkaline information. Commercial litmus may be standardized.

Universal indicator

Universal indicator

Universal indicator is a mixture producing a continuous color scale from red to violet across pH 1–14. Ideal for visual pH mapping and teaching. It provides qualitative pH values; paper strips often more convenient for field use.

Methyl red

Methyl red

Methyl red goes from red in acid to yellow in base between pH 4.4 and 6.2. Used in microbiology MR tests and specific titrations where a low-range endpoint is needed. Toxicity is low but avoid ingestion and skin contact.

Thymol blue

Thymol blue

Thymol blue has two distinct color jumps: red→yellow at very low pH and yellow→blue in the alkaline range. Useful when either low or high pH endpoints are required. It’s light-sensitive; store in dark containers.

Bromocresol green

Bromocresol green

Bromocresol green shifts from yellow in acid to blue-green in base around pH 3.8–5.4. Often used in clinical assays and pH papers for low-to-mid range monitoring. Handle with care—avoid inhalation of powder.

Phenol red

Phenol red

Phenol red changes from yellow to red across pH 6.8–8.4 and is common in cell culture media, water testing and aquaria. It provides a useful physiological-range indicator but can interact with some assays; non-toxic at working concentrations.

Congo red

Congo red

Congo red shifts from blue to red in acidic conditions near pH 3–5 and is famous for staining amyloid in biology. As an indicator it gives visual acidic transitions but is a larger dye with known handling precautions; avoid inhalation.

Nitrazine yellow

Nitrazine yellow

Nitrazine yellow is used in disposable paper tests to detect pH changes between pH 5 and 8. It is fast and practical for clinical screening (for example, ruptured membranes). Use single-use strips and follow clinical guidelines.

Alizarin yellow R

Alizarin yellow R

Alizarin yellow R acts as a high-range pH indicator and complexes with certain metal ions. It shifts color in strongly alkaline solutions and can serve as a metallochromic reagent; handle as a chemical irritant and avoid release to drains.

Thymolphthalein

Thymolphthalein

Thymolphthalein is colorless below pH ~9.3 and turns deep blue above pH 10. Useful for high-pH titrations where phenolphthalein is fading. Store away from light and strong acids; prepare in alcohol solutions for titrations.

Eriochrome Black T

Eriochrome Black T

Eriochrome Black T is the classic EDTA titration indicator: wine-red free dye turns blue when bound to metal; endpoint occurs when EDTA displaces the indicator, producing a clear color jump. Use buffered samples; dye is irritant.

Calmagite

Calmagite

Calmagite is a metallochromic indicator used for calcium and magnesium EDTA titrations, changing color upon complex formation. It offers alternatives to Eriochrome Black T with similar handling needs; avoid skin contact and inhalation.

Murexide

Murexide

Murexide (ammonium purpurate) produces distinct purplish complexes with calcium and other metals, used in classical calcium assays and gravimetric checks. It’s light-sensitive and must be handled and disposed of responsibly due to potential toxicity.

Alizarin complexone

Alizarin complexone

Alizarin complexone is a sensitive indicator for calcium and other metals in EDTA titrations; it produces clear color transitions and works across a useful pH window. Store dry and use buffers for consistent endpoints.

PAR (4-(2-pyridylazo)resorcinol)

PAR (4-(2-pyridylazo)resorcinol)

PAR reacts with transition metals to give vivid colors and is used for quantitative and qualitative metal assays. It’s versatile for labs and field tests; handle with gloves and avoid contamination of samples.

Xylenol orange

Xylenol orange

Xylenol orange is a metallochromic indicator useful for trace metal EDTA titrations; it forms colored complexes with many metal ions. It’s sensitive and commonly used where spectrophotometric follow-up is possible; keep protected from light.

Ferroin

Ferroin

Ferroin (iron(II)-1,10-phenanthroline complex) is a robust redox indicator giving a strong red-to-pale-blue change on oxidation. It’s widely used in redox titrimetry for clear endpoints; store under inert conditions to avoid air oxidation.

Dichlorophenolindophenol (DCPIP)

Dichlorophenolindophenol (DCPIP)

DCPIP is a blue redox dye that becomes colorless when reduced; commonly used to titrate vitamin C and to demonstrate photosynthetic electron transport. It’s easy-to-see and works in aqueous solutions; avoid ingestion and prolonged skin contact.

Starch (iodine-starch complex)

Starch (iodine-starch complex)

Starch acts as an indicator by forming an intense blue-black complex with iodine or triiodide; added near the endpoint of iodometric titrations for high sensitivity. Use only near endpoint to avoid destroyed complexes; prepare fresh solutions.

Methylene blue

Methylene blue

Methylene blue is a classic redox indicator that loses color when reduced, used in redox studies and as a simple oxygen indicator. It is also used in small-scale biological tests; avoid skin staining and follow waste disposal rules.

Diphenylamine

Diphenylamine

Diphenylamine develops a blue color when oxidized by strong oxidizers such as nitrates in acidic solution; used in qualitative nitrate tests and explosives screening. Toxic and potentially hazardous—use minimal amounts, proper ventilation and protective equipment.

Resazurin

Resazurin

Resazurin is a blue dye that becomes pink then colorless on reduction; used as a viability/respirometry indicator in microbiology and packaged-food freshness tests. Non-destructive at low concentrations; follow biolab safety guidance.

Fluorescein (Fajans adsorption)

Fluorescein (Fajans adsorption)

Fluorescein serves as an adsorption indicator in Fajans titrations: the dye adsorbs on a precipitate (e.g., AgCl), producing a sharp color change or fluorescence at the endpoint. Use dilute solutions and avoid prolonged light exposure.

Potassium chromate (Mohr method)

Potassium chromate (Mohr method)

In the Mohr method, chromate acts as an indicator ion producing red silver chromate precipitate when all chloride has been precipitated. It’s simple and visual but requires neutral–slightly basic conditions; chromate is toxic and carcinogenic—handle with extreme care.

Phenolphthalein-methyl orange mixed strips

Phenolphthalein-methyl orange mixed strips

Combined indicator strips use sequenced dyes to give multi-color pH readouts across wide ranges. They’re disposable, fast, and user-friendly for general pH checks. Results are semi-quantitative—confirm with calibrated meters for precision work.

Alizarin S

Alizarin S

Alizarin S gives vivid color changes on metal binding, often used for aluminum and other metal assays. It’s valuable in complexometric titrations and spot tests; handle as a chemical irritant and avoid environmental release.

PAN (1-(2-pyridylazo)-2-naphthol)

PAN (1-(2-pyridylazo)-2-naphthol)

PAN forms bright colored complexes with many transition metals and is used for rapid colorimetric identification or simple field assays. Useful for quick screening, but some metal specificity and safety precautions for solvent use apply.

Curcumin (turmeric)

Curcumin (turmeric)

Curcumin from turmeric changes from yellow to reddish-brown in alkaline solutions, providing a natural pH indicator for demonstrations and simple home tests. Non-toxic in small amounts and popular in teaching, though less precise than lab indicators.

Red cabbage extract (anthocyanins)

Red cabbage extract (anthocyanins)

Red cabbage anthocyanins produce a rainbow of colors across the pH scale, making them excellent for visual demonstrations and DIY pH strips. Non-toxic and inexpensive, but not as stable or specific as synthetic indicators.

Beetroot (betalains)

Beetroot (betalains)

Beet pigments change hue and can bleach under oxidative conditions; useful for simple demonstrations of redox and pH effects in foods and classrooms. Edible and safe, but colors fade with heat and light.

Ferricyanide/ferrocyanide redox indicators

Ferricyanide/ferrocyanide redox indicators

Ferricyanide/ferrocyanide couples exhibit observable color shifts in solution depending on oxidation state and are used in redox demonstrations and sensors. They give clear visual cues for electron transfer but require care: cyanide complexes demand proper handling and disposal.

Tollen's reagent (silver mirror)

Tollen’s reagent (silver mirror)

Tollen’s reagent produces a silver mirror or black precipitate when aldehydes reduce Ag+. This dramatic visual test is a classic qualitative assay; it requires careful handling and immediate, safe disposal due to silver and ammonia content.

Stannous chloride/iodine test

Stannous chloride/iodine test

Stannous chloride is used in spot-tests to reduce or precipitate specific species; visible color or precipitate changes signal redox chemistry. Useful in analytical spot tests but requires control of acid and reductant concentrations for clear endpoints.

Fluorescent pH indicators (e.g., SNARF dyes)

Fluorescent pH indicators (e.g., SNARF dyes)

Fluorescent pH dyes change emission color or intensity with pH and are powerful for imaging intracellular pH. They require fluorescence equipment to read but provide high spatial resolution; do not include instrument-only sensors without visible color change.

Lead(II) chromate (spot tests)

Lead(II) chromate (spot tests)

Some classical spot-test reagents produce colored precipitates with target ions usable for quick field screening. They give immediate visual confirmation but are qualitative and may involve toxic heavy metals—use extreme care and prefer safer modern alternatives.

Oxazine dyes (redox)

Oxazine dyes (redox)

Oxazine-family dyes undergo visually obvious color shifts on redox changes and serve as indicators in some biochemical and analytical methods. They are sensitive and useful where colorless reduced forms are needed; handle with standard lab precautions.

Xylenol blue (sulfonephthalein type)

Xylenol blue (sulfonephthalein type)

Xylenol-type dyes change color in the physiological to mildly alkaline range and are used in buffer checks and some clinical applications. They are suited to solutions where neutral-to-slightly-basic endpoints are relevant; consult supplier data for exact ranges.

Coomassie Brilliant Blue (protein dye with pH sensitivity)

Coomassie Brilliant Blue (protein dye with pH sensitivity)

Coomassie dye shows a measurable color change upon protein binding used in Bradford assays; it’s an example of a dye acting as an indicator of biochemical presence rather than pure pH. Follow waste disposal guidelines; hazardous in concentrated form.